pPOTv6-puro-3Ty:mNG:3Ty
(Plasmid
#179798)
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PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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Depositing Labs
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Depositing OrganizationUniversity of Oxford
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 179798-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 179798-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPOT
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNG
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SpeciesSynthetic
- Promoter read through
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer cTATGGGTTCTGGTAGTG
- 3′ sequencing primer cTTAACTACCCGATCCTG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
DNA (Catalog # 179798-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 179798-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOTv6-puro-3Ty:mNG:3Ty was a gift from Samuel Dean & Keith Gull (Addgene plasmid # 179798 ; http://n2t.net/addgene:179798 ; RRID:Addgene_179798) -
For your References section:
A comprehensive toolkit for protein localization and functional analysis in trypanosomatids. Paterou A, Saez Conde J, Tyc J, Sunter JD, Vaughan S, Gull K, Dean S. Open Biol. 2025 Apr;15(4):240361. doi: 10.1098/rsob.240361. Epub 2025 Apr 2. 10.1098/rsob.240361 PubMed 40169015
