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Unlocking Trypanosome Biology: A Comprehensive Protein-Tagging Toolkit for Localization and Functional Analysis
Paterou A, Tyc J, Sunter J, Vaughan S, Gull K, Dean S
bioRxiv 2023.04.21.537815
Article

Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:

  • Samuel Dean
  • Keith Gull
  • Jack Sunter
  • Sue Vaughan
  • Plasmids from Article

    ID Plasmid Purpose  
    179781pPOTv7-blast-eGFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179782pPOTv7-blast-sfGFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179783pPOTv6-blast-3Ty:mNG:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179784pPOTv7-blast-eYFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179785pPOTv7-blast-mNG:HaloTagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179786pPOTv7-blast-SNAPtagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179787pPOTv7-blast-CLIPtagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179788pPOTv7-blast-birA*Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179789pPOTv7-blast-mCherryTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179790pPOTv7-blast-tagRFPtTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179791pPOTv7-blast-mTurquoise2Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179792pPOTv7-blast-mTFP1Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179793pPOTv7-blast-mRuby3Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179794pPOTv7-blast-tdTomatoTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179795pPOTv7-blast-lssmOrangeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179796pPOTv7-blast-mCardinalTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179797pPOTv7-blast-nanolucTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179798pPOTv6-puro-3Ty:mNG:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179799pPOTv6-hygro-3Ty:mNG:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179800pPOTv6-g418-3Ty:mNG:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179801pPOTv6-blast-3Myc:mNG:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179802pPOTv6-blast-3Myc:mScarletI:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179803pPOTv6-puro-3Myc:mNG:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179804pPOTv6-puro-3Myc:mScarletI:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179805pPOTv6-blast-3Ty:syPher2:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179806pPOTv6-blast-3Ty:pHlourin2:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179807pPOTv7-blast-10TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179808pPOTv7-hygro-10TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179809pPOTv7-hygro-10V5Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179810pPOTv7-g418-10V5Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179811pPOTv7-g418-10MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179812pPOTv7-g418-10FLAGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179813pPOTv7-g418-10HATrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179814pPOTv7-hygro-10MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179815pPOTv7-hygro-10FLAGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179816pPOTv7-hygro-10HATrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179817pPOTv7-blast-mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179818pPOTv7-blast-mScarletTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179819pPOTv7-hygro-mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179820pPOTv7-hygro-mScarletITrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179821pPOTv7-g418-mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179822pPOTv7-g418-mScarletITrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179823pPOTv7-g418-mScarletTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179824pPOTv6-blast-3Ty:pHlourinM153R:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179827pPOTv7-blast-3HaloTagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179828pPOTv7-hygro-3HaloTagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179829pPOTv7-g418-3HaloTagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179831pPOTv7-blast-3mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179832pPOTv7-g418-3mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    179833pPOTv7-hygro-3mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181935pPOTv7.1-phleo-mNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181936pPOTv6.1-phleo-Ty:mNG:TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181937pPOTv6.1-phleo-3Myc:mNG:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181938pPOTv7-blast-10V5Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181939pPOTv7-blast-10MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181940pPOTv7-blast-10FLAGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181941pPOTv7-blast-10HATrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181942pPOTv6-blast-3Ty:HaloTag:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181943pPOTv6-blast-3Myc:HaloTag:3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    181953pPOTv7-blast-HaloTagTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190206pPOTv7-blast-sfCherry2(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190207pPOTv7-blast-sfCherry2(11)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190208pPOTv7-blast-spGFP(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190209pPOTv7-blast-mNG2(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190210pPOTv7-blast-mNG2(11)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190211pPOTv7-g418-sfCherry2(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190212pPOTv7-g418-sfCherry2(11)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190213pPOTv7-g418-spGFP(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190214pPOTv7-g418-mNG2(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190215pPOTv7-g418-mNG2(11)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190216pPOTv7-hygro-mNG2(1-10)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190217pPOTv6-g418-3Myc-mNG-3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190218pPOTv6-hygro-3Myc-mNG-3MycTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190219pPOTv7-hygro-eYFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190220pPOTv7-g418-eYFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190221pPOTv7-blast-mGLTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190222pPOTv7-blast-moxNGTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190257pPOTv7-g418-spGFP(11)Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190258pPOTv7-blast-BFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190259pPOTv7-g418-BFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190260pPOTv7-hygro-BFPTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190261pPOTv7-g418-mCherryTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190262pPOTv7-hygro-mCherryTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    190263pPOTv7-hygro-mScarletTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201084pPOTv7-hygro-tagRFPtTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201085pPOTv7-g418-tagRFPtTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201086pPOTv6-blast-3Ty:tagRFPt:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201087pPOTv6-g418-3Ty:tagRFPt:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201088pPOTv6-hygro-3Ty:tagRFPt:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201089pPOTv6-puro-3Ty:tagRFPt:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201090pPOTv6-blast-3Ty:mScarletI:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201091pPOTv6-g418-3Ty:mScarletI:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201092pPOTv6-hygro-3Ty:mScarletI:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201093pPOTv6-puro-3Ty:mScarletI:3TyTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201095pPOTv6-g418-His:mNG:HisTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201097pPOTv6-blast-His:mScarletI:HisTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201098pPOTv6-g418-His:mScarletI:HisTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201441pPOTv7-blast-mNG:Ppyr9ehTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201442pPOTv7-blast-mScarletITrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    201443pPOTv7-blast-Ppyr9ehTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging
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    Recombinant Antibodies from Article