Unlocking Trypanosome Biology: A Comprehensive Protein-Tagging Toolkit for Localization and Functional Analysis
Paterou A, Tyc J, Sunter J, Vaughan S, Gull K, Dean S
bioRxiv 2023.04.21.537815 Article
Paterou A, Tyc J, Sunter J, Vaughan S, Gull K, Dean S
bioRxiv 2023.04.21.537815 Article
Plasmids from this article have been deposited by multiple labs. See below for all materials from each lab:
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179781 | pPOTv7-blast-eGFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179782 | pPOTv7-blast-sfGFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179783 | pPOTv6-blast-3Ty:mNG:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179784 | pPOTv7-blast-eYFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179785 | pPOTv7-blast-mNG:HaloTag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179786 | pPOTv7-blast-SNAPtag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179787 | pPOTv7-blast-CLIPtag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179788 | pPOTv7-blast-birA* | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179789 | pPOTv7-blast-mCherry | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179790 | pPOTv7-blast-tagRFPt | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179791 | pPOTv7-blast-mTurquoise2 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179792 | pPOTv7-blast-mTFP1 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179793 | pPOTv7-blast-mRuby3 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179794 | pPOTv7-blast-tdTomato | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179795 | pPOTv7-blast-lssmOrange | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179796 | pPOTv7-blast-mCardinal | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179797 | pPOTv7-blast-nanoluc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179798 | pPOTv6-puro-3Ty:mNG:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179799 | pPOTv6-hygro-3Ty:mNG:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179800 | pPOTv6-g418-3Ty:mNG:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179801 | pPOTv6-blast-3Myc:mNG:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179802 | pPOTv6-blast-3Myc:mScarletI:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179803 | pPOTv6-puro-3Myc:mNG:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179804 | pPOTv6-puro-3Myc:mScarletI:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179805 | pPOTv6-blast-3Ty:syPher2:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179806 | pPOTv6-blast-3Ty:pHlourin2:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179807 | pPOTv7-blast-10Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179808 | pPOTv7-hygro-10Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179809 | pPOTv7-hygro-10V5 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179810 | pPOTv7-g418-10V5 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179811 | pPOTv7-g418-10Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179812 | pPOTv7-g418-10FLAG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179813 | pPOTv7-g418-10HA | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179814 | pPOTv7-hygro-10Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179815 | pPOTv7-hygro-10FLAG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179816 | pPOTv7-hygro-10HA | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179817 | pPOTv7-blast-mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179818 | pPOTv7-blast-mScarlet | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179819 | pPOTv7-hygro-mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179820 | pPOTv7-hygro-mScarletI | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179821 | pPOTv7-g418-mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179822 | pPOTv7-g418-mScarletI | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179823 | pPOTv7-g418-mScarlet | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179824 | pPOTv6-blast-3Ty:pHlourinM153R:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179827 | pPOTv7-blast-3HaloTag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179828 | pPOTv7-hygro-3HaloTag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179829 | pPOTv7-g418-3HaloTag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179831 | pPOTv7-blast-3mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179832 | pPOTv7-g418-3mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 179833 | pPOTv7-hygro-3mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181935 | pPOTv7.1-phleo-mNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181936 | pPOTv6.1-phleo-Ty:mNG:Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181937 | pPOTv6.1-phleo-3Myc:mNG:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181938 | pPOTv7-blast-10V5 | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181939 | pPOTv7-blast-10Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181940 | pPOTv7-blast-10FLAG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181941 | pPOTv7-blast-10HA | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181942 | pPOTv6-blast-3Ty:HaloTag:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181943 | pPOTv6-blast-3Myc:HaloTag:3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 181953 | pPOTv7-blast-HaloTag | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190206 | pPOTv7-blast-sfCherry2(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190207 | pPOTv7-blast-sfCherry2(11) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190208 | pPOTv7-blast-spGFP(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190209 | pPOTv7-blast-mNG2(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190210 | pPOTv7-blast-mNG2(11) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190211 | pPOTv7-g418-sfCherry2(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190212 | pPOTv7-g418-sfCherry2(11) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190213 | pPOTv7-g418-spGFP(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190214 | pPOTv7-g418-mNG2(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190215 | pPOTv7-g418-mNG2(11) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190216 | pPOTv7-hygro-mNG2(1-10) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190217 | pPOTv6-g418-3Myc-mNG-3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190218 | pPOTv6-hygro-3Myc-mNG-3Myc | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190219 | pPOTv7-hygro-eYFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190220 | pPOTv7-g418-eYFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190221 | pPOTv7-blast-mGL | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190222 | pPOTv7-blast-moxNG | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190257 | pPOTv7-g418-spGFP(11) | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190258 | pPOTv7-blast-BFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190259 | pPOTv7-g418-BFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190260 | pPOTv7-hygro-BFP | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190261 | pPOTv7-g418-mCherry | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190262 | pPOTv7-hygro-mCherry | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 190263 | pPOTv7-hygro-mScarlet | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201084 | pPOTv7-hygro-tagRFPt | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201085 | pPOTv7-g418-tagRFPt | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201086 | pPOTv6-blast-3Ty:tagRFPt:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201087 | pPOTv6-g418-3Ty:tagRFPt:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201088 | pPOTv6-hygro-3Ty:tagRFPt:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201089 | pPOTv6-puro-3Ty:tagRFPt:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201090 | pPOTv6-blast-3Ty:mScarletI:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201091 | pPOTv6-g418-3Ty:mScarletI:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201092 | pPOTv6-hygro-3Ty:mScarletI:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201093 | pPOTv6-puro-3Ty:mScarletI:3Ty | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201095 | pPOTv6-g418-His:mNG:His | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201097 | pPOTv6-blast-His:mScarletI:His | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201098 | pPOTv6-g418-His:mScarletI:His | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201441 | pPOTv7-blast-mNG:Ppyr9eh | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201442 | pPOTv7-blast-mScarletI | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |
| 201443 | pPOTv7-blast-Ppyr9eh | Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging |