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Depositing Lab
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Depositing OrganizationGeisinger Health Center
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 18068-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 18068-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMIK-Neo
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Backbone manufacturergift from Japan
- Backbone size w/o insert (bp) 6200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyes kinase
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Alt namec-yes
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SpeciesM. musculus (mouse)
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Insert Size (bp)1800
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MutationTyrosine 535 changed to Phenylalanine
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GenBank IDNM_009535
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Entrez GeneYes1 (a.k.a. Yes, p61-Yes)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (destroyed during cloning)
- 3′ cloning site MluI (destroyed during cloning)
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe mouse "yes" cDNA gene was a gift from Dr. J. Bolen (Oncogene 1993, Volume 8, pages 713-719, the first author: Klages, S.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The vector pMIK-Neo is a gift from Dr. Kazuo Maruyama of the University of Tokyo, Tokyo, Japan. The map of the vector will be sent with the DNA
DNA (Catalog # 18068-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 18068-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIK-Neo-mYes-Y535F was a gift from Marius Sudol (Addgene plasmid # 18068 ; http://n2t.net/addgene:18068 ; RRID:Addgene_18068) -
For your References section:
Yes-associated protein and p53-binding protein-2 interact through their WW and SH3 domains. Espanel X, Sudol M. J Biol Chem. 2001 Apr 27. 276(17):14514-23. 10.1074/jbc.M008568200 PubMed 11278422
Map uploaded by the depositor.
