p2xFlag CMV2 Lats1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18971||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep2xFlag CMV2
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLarge tumor suppressor kinase 1
SpeciesH. sapiens (human)
Insert Size (bp)3393
Entrez GeneLATS1 (a.k.a. WARTS, wts)
- Promoter CMV
/ Fusion Protein
- 2xFlag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
*note that a user discovered a S1111L mutation in this insert. The origin of this substitution is unknown, as is its effect on function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2xFlag CMV2 Lats1 was a gift from Marius Sudol (Addgene plasmid # 18971)
For your References section:Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976
Map uploaded by the depositor.