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pαH-rS2d-HexaPro
(Plasmid #183515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183515 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 183515-D50 50 μg of DNA in Tris buffer $495
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DNA 183515-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (S-GSAS-rS2d.HexaPro)
  • Alt name
    pαH-S-GSAS-rS2d.HexaPro
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3624
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • HRV3C cleavage site (C terminal on backbone)
    • 8X His tag (C terminal on backbone)
    • 2X Strep-Tag II (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from a plasmid made by Jason McLellan; https://www.addgene.org/164566/.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-rS2d-HexaPro was a gift from Priyamvada Acharya (Addgene plasmid # 183515 ; http://n2t.net/addgene:183515 ; RRID:Addgene_183515)

  • For your References section:

    Structural diversity of the SARS-CoV-2 Omicron spike. Gobeil SM, Henderson R, Stalls V, Janowska K, Huang X, May A, Speakman M, Beaudoin E, Manne K, Li D, Parks R, Barr M, Deyton M, Martin M, Mansouri K, Edwards RJ, Eaton A, Montefiori DC, Sempowski GD, Saunders KO, Wiehe K, Williams W, Korber B, Haynes BF, Acharya P. Mol Cell. 2022 Jun 2;82(11):2050-2068.e6. doi: 10.1016/j.molcel.2022.03.028. Epub 2022 Mar 25. 10.1016/j.molcel.2022.03.028 PubMed 35447081
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