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pT22U6CatalE4PCh(#767)
(Plasmid #184078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 184078 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT22i (pT2AL200R150G derivative)
  • Backbone manufacturer
    modified by M Volovitch from pT2AL200R150 of A Urasaki; Urasaki et al 2006, Genetics 174:639)
  • Backbone size w/o insert (bp) 8082
  • Vector type
    Zebrafish transgenesis (blue eyes) + in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CatalDeltaC-ERT2-P2A-mCherry
  • Species
    M. musculus (CatalDeltaC), H. sapiens (ERT2), PTV-1 (P2A), Discosoma sp. (mCherry)
  • Insert Size (bp)
    3348
  • Mutation
    CatalDeltaC: deleted 516-527; ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Zf-Ubi+SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SVLrev34
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic ERT2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CatalDeltaC: NP_033934; ERT2: NP_000116; P2A: AIE57569; mCherry: AY678264 (polycistronic mRNA: mCherry cytoplasmic marker of expression) + gCRY:CFP as a marker of transgenesis;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT22U6CatalE4PCh(#767) was a gift from Sophie Vriz (Addgene plasmid # 184078 ; http://n2t.net/addgene:184078 ; RRID:Addgene_184078)