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pCSThaP6ERT(#184)
(Plasmid #184103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184103 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 184103-D50 50 μg of DNA in Tris buffer $495
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DNA 184103-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCST (pCS2 derivative)
  • Backbone manufacturer
    M Volovitch
  • Backbone size w/o insert (bp) 4223
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-MmPax6-ERT2
  • Species
    Influenza A virus (HA), M. musculus (Pax6), H. sapiens (ERT2)
  • Insert Size (bp)
    2304
  • Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter sCMV+SP6+T7
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site BsaBI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SVLrev34
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA; MmPax6: NP_001231130; ERT2: NP_000116;

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSThaP6ERT(#184) was a gift from Sophie Vriz (Addgene plasmid # 184103 ; http://n2t.net/addgene:184103 ; RRID:Addgene_184103)
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