PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78328||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepInducer20 (Addgene plasmid #44012)
Backbone manufacturerStephen Elledge
- Backbone size w/o insert (bp) 11791
- Total vector size (bp) 12791
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Alt nameMyogenic differentiation 1
SpeciesH. sapiens (human)
Insert Size (bp)1000
Entrez GeneMYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
- Promoter TRE2
/ Fusion Protein
- 3x HA tag (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CAAGGATGACGACGATAAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Validated in conversion of fibroblasts to myoblasts. Please contact Dr. Alexander if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pINDUCER20-hMYOD1-3xHA (#730) was a gift from Matthew Alexander & Louis Kunkel (Addgene plasmid # 78328 ; http://n2t.net/addgene:78328 ; RRID:Addgene_78328)
Map uploaded by the depositor.