pSP64T acvr1ba*
(Plasmid
#185409)
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PurposeConstitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP64T
- Backbone size w/o insert (bp) 3240
- Total vector size (bp) 4891
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Vector typeMammalian Expression ; In vitro synthesis of mRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameacvr1ba
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Alt nameacvr1b, taram-a, tarama, alk4
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SpeciesD. rerio (zebrafish)
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Mutationchanged Threonine 206 to Aspartic acid
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Entrez Geneacvr1ba (a.k.a. acvr1b, alk4, axis, taram-a, tarama, wu:fb75f01)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64T acvr1ba* was a gift from Nicolas David (Addgene plasmid # 185409 ; http://n2t.net/addgene:185409 ; RRID:Addgene_185409) -
For your References section:
Guidance by followers ensures long-range coordination of cell migration through alpha-catenin mechanoperception. Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss J, Brusch L, David NB. Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001. 10.1016/j.devcel.2022.05.001 PubMed 35613615
Map uploaded by the depositor.
