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PurposeRNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePB533
- Total vector size (bp) 8351
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name42B3mutAC2-scFv
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Alt name42B3(R78K/A80T/M95V)-scFv
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Insert Size (bp)734
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GenBank IDLC628457
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Tag
/ Fusion Protein
- sfGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB533-42B3mutAC2-sfGFP was a gift from Hiroshi Kimura (Addgene plasmid # 186777 ; http://n2t.net/addgene:186777 ; RRID:Addgene_186777) -
For your References section:
Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. Uchino S, Ito Y, Sato Y, Handa T, Ohkawa Y, Tokunaga M, Kimura H. J Cell Biol. 2022 Feb 7;221(2). pii: 212888. doi: 10.1083/jcb.202104134. Epub 2021 Dec 2. 10.1083/jcb.202104134 PubMed 34854870
Map uploaded by the depositor.
