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PurposeH3K27me3-mintbody expression vector for mammalian cells
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePB533
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2E12LI-scFv
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Insert Size (bp)746
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Tag
/ Fusion Protein
- sfGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB533_2E12LI-sfGFP was a gift from Hiroshi Kimura (Addgene plasmid # 167527 ; http://n2t.net/addgene:167527 ; RRID:Addgene_167527) -
For your References section:
H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. Tjalsma SJD, Hori M, Sato Y, Bousard A, Ohi A, Raposo AC, Roensch J, Le Saux A, Nogami J, Maehara K, Kujirai T, Handa T, Bages-Arnal S, Ohkawa Y, Kurumizaka H, da Rocha ST, Zylicz JJ, Kimura H, Heard E. EMBO Rep. 2021 Feb 19:e51989. doi: 10.15252/embr.202051989. 10.15252/embr.202051989 PubMed 33605056
Map uploaded by the depositor.
