pCS2+ Zf GFP-vcla
(Plasmid
#185410)
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PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV
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Depositing Lab
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Depositing OrganizationCNRS UMR 7645, Laboratoire d'Optique et Biosciences, Ecole Polytechnique
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 185410-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 185410-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 8035
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Vector typeMammalian Expression ; In vitro synthesis of mRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevinculin a
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Alt namevcla, vcl
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3945
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Entrez Genevcla (a.k.a. hm:zehn2160, im:6902790, vcl)
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Tag
/ Fusion Protein
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Johan de Rooij, University Medical Center Utrecht
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.
DNA (Catalog # 185410-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 185410-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ Zf GFP-vcla was a gift from Nicolas David (Addgene plasmid # 185410 ; http://n2t.net/addgene:185410 ; RRID:Addgene_185410) -
For your References section:
Guidance by followers ensures long-range coordination of cell migration through alpha-catenin mechanoperception. Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss J, Brusch L, David NB. Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001. 10.1016/j.devcel.2022.05.001 PubMed 35613615
Map uploaded by the depositor.
