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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7
(Plasmid #187582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187582 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 187582-D50 50 μg of DNA in Tris buffer $495
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DNA 187582-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRSF7
  • Species
    H. sapiens (human)
  • Entrez Gene
    SRSF7 (a.k.a. 9G8, AAG3, SFRS7)
  • Promoter CMV
  • Tag / Fusion Protein
    • APEX2-FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA Gateway entry clone for this plasmid was provided by Dr. Anne-Claude Gingras' laboratory at Lunenfeld-Tanenbaum Research Institute.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7 was a gift from Benjamin Blencowe (Addgene plasmid # 187582 ; http://n2t.net/addgene:187582 ; RRID:Addgene_187582)

  • For your References section:

    Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns. Barutcu AR, Wu M, Braunschweig U, Dyakov BJA, Luo Z, Turner KM, Durbic T, Lin ZY, Weatheritt RJ, Maass PG, Gingras AC, Blencowe BJ. Mol Cell. 2022 Mar 3;82(5):1035-1052.e9. doi: 10.1016/j.molcel.2021.12.010. Epub 2022 Feb 18. 10.1016/j.molcel.2021.12.010 PubMed 35182477
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