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Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns.
Barutcu AR, Wu M, Braunschweig U, Dyakov BJA, Luo Z, Turner KM, Durbic T, Lin ZY, Weatheritt RJ, Maass PG, Gingras AC, Blencowe BJ
Mol Cell. 2022 Mar 3;82(5):1035-1052.e9. doi: 10.1016/j.molcel.2021.12.010. Epub 2022 Feb 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
182925pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-termExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.
182926pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-termExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.
187574pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells
187575pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells
187576pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNAExpress FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells
187577pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBLExpress FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells
187578pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells
187579pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells
187580pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONOExpress FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells
187581pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILINExpress FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells
187582pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells
187583pcDNA5-FRT/TO-3*Flag-APEX2 C-term PMLExpress FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells
187584pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells
187585pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPATExpress FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells
187586pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells
187587pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells
187588pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLSExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Antibodies from Article