pFastBac_Dual_GST-TEV-EGFP-TBK1
(Plasmid #187830)

• Purpose: Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631
• Depositing Lab: Sascha Martens
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pFastBac_Dual
• Backbone manufacturer: Thermofisher Scientific
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 8776
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TBK1
• Alt name: NAK
• Species: H. sapiens (human)
• Insert Size (bp): 3625
• GenBank ID: 29110 NM_013254
• Entrez Gene: TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • TEV-mEGFP-GG Linker (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGAGGCATGCAATCCACATCCAACCACC
• 3′ sequencing primer: CCTCTAGTACTTCTCGACAAGCTTTTACAGACAGTCCACGTTTCTCAGT

Resource Information

• Supplemental Documents:
  • SMC1631 - . pFastBac_GST-TEV-eGFP-TBK1_new-cloning-attempt.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Codon Optimized for Sf9 Expression