Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M
Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. PubMed Article
Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M
Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. PubMed Article
Plasmids from this article have been deposited by two labs. See below for all materials from each lab:
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186221 | pBMN BFP-Parkin | For expression of Parkin tagged with BFP |
| 186222 | pBMN HA-ATG13(Δ1-198) | For expression of ATG13(Δ1-198) tagged with HA |
| 186223 | pBMN HA-ATG13 | For expression of ATG13 tagged with HA |
| 187827 | pGST2-GST-TEV-OPTN S177D S473D | Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588 |
| 187828 | pGST2-GST-TEV-NDP52 | Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586 |
| 187829 | pETDuet-1_6xHis-TEV-mCh-NDP52 | Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148 |
| 187830 | pFastBac_Dual_GST-TEV-EGFP-TBK1 | Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631 |
| 187936 | pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 | Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327 |
| 188643 | pBMN mEGFP | This is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genes |
| 188644 | pBMN BFP | This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes |
| 188645 | pBMN HA | This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes |
| 188784 | pBMN mEGFP-OPTN | For mammalian expression of OPTN tagged with mEGFP |
| 188785 | pBMN mEGFP-NDP52 | For mammalian expression of NDP52 tagged with mEGFP |
| 198033 | pFastBac-GST-TEV-mCherry-TBK1 | Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675) |
| 198034 | pCAGG-GST-TEV-NAP1 (WT) | Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064) |
| 198035 | pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9) | Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613) |
| 198036 | pCAGG-GST-TEV-mCherry-NAP1 (WT) | Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065) |
| 198037 | pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K | Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649) |
| 198072 | pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666) | Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874) |
| 198428 | pGST2-GST-TEV-OPTN | Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587) |
| 199205 | pBMN-HA-TBK1 | Used for the expression of TBK1 (SMC Internal No. 1996) |
| 199206 | pBMN_HA-TBK1 (kinase dead - D135N) | Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997) |
| 199207 | pBMN_HA-TBK1 (E696K) | Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998) |
| 199307 | pBMN HA-NDP52 | For expression of the mammalian NDP52 tagged with HA |