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pBMN_HA-TBK1 (kinase dead - D135N)
(Plasmid #199206)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 199206 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone size w/o insert (bp) 5029
  • Total vector size (bp) 7216
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TBK1
  • Alt name
    NF-kappa-B-activating kinase
  • Alt name
    T2K
  • Alt name
    NAK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2187
  • Mutation
    D135N
  • GenBank ID
    AF191838
  • Entrez Gene
    TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATGGTATAGTGCACCGTAATATCAAGCCAGGAAAT
  • 3′ sequencing primer TCCTAAAGACAGTCAACGTTGCGAAGGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN_HA-TBK1 (kinase dead - D135N) was a gift from Sascha Martens (Addgene plasmid # 199206 ; http://n2t.net/addgene:199206 ; RRID:Addgene_199206)
  • For your References section:

    Unconventional initiation of PINK1/Parkin mitophagy by Optineurin. Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn M, Lindblom RSJ, Martens S, Lazarou M. Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021. 10.1016/j.molcel.2023.04.021 PubMed 37207627