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pHAGE-N-FLAG-HA-TBK1
(Plasmid #131791)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 131791 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE-N-FLAG-HA-IRES-PURO
  • Backbone manufacturer
    Gateway, backbone originally from J.Wade Harper lab
  • Backbone size w/o insert (bp) 9613
  • Total vector size (bp) 9978
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TBK1 WT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2190
  • Mutation
    WT full length
  • GenBank ID
    29110
  • Entrez Gene
    TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
  • Promoter CMV
  • Tag / Fusion Protein
    • N-FLAG-HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SAS109 acatatagacaaacgcac
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-N-FLAG-HA-TBK1 was a gift from Alicia Pickrell (Addgene plasmid # 131791 ; http://n2t.net/addgene:131791 ; RRID:Addgene_131791)
  • For your References section:

    PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, Artavanis-Tsakonas S, Youle RJ, Pickrell AM. Cell Rep. 2019 Oct 1;29(1):225-235.e5. doi: 10.1016/j.celrep.2019.08.085. 10.1016/j.celrep.2019.08.085 PubMed 31577952