pHAGE-N-FLAG-HA-TBK1
(Plasmid #131791)

• Purpose: Lentiviral vector expressing WT TBK1
• Depositing Lab: Alicia Pickrell
• Publication: Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. doi: 10.1016/j.celrep.2019.08.085.

Backbone

• Vector backbone: pHAGE-N-FLAG-HA-IRES-PURO
• Backbone manufacturer: Gateway, backbone originally from J.Wade Harper lab
• Backbone size w/o insert (bp): 9613
• Total vector size (bp): 9978
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TBK1 WT
• Species: H. sapiens (human)
• Insert Size (bp): 2190
• Mutation: WT full length
• GenBank ID: 29110
• Entrez Gene: TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
• Promoter: CMV
• Tag / Fusion Protein:
  • N-FLAG-HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SAS109 acatatagacaaacgcac

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry