|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32713||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneIRF3 (a.k.a. IIAE7)
- Promoter CMV
/ Fusion Protein
- V5 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human V5-IRF3-pcDNA3 was a gift from Saumen Sarkar (Addgene plasmid # 32713 ; http://n2t.net/addgene:32713 ; RRID:Addgene_32713)
For your References section:High-throughput screening for TLR3-IFN regulatory factor 3 signaling pathway modulators identifies several antipsychotic drugs as TLR inhibitors. Zhu J, Smith K, Hsieh PN, Mburu YK, Chattopadhyay S, Sen GC, Sarkar SN. J Immunol. 2010 May 15;184(10):5768-76. Epub 2010 Apr 9. 10.4049/jimmunol.0903559 PubMed 20382888