pGST2-GST-TEV-OPTN S177D S473D
(Plasmid #187827)

• Purpose: Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588
• Depositing Lab: Sascha Martens
• Publication: Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 10.1016/j.molcel.2023.04.021.

Backbone

• Vector backbone: pGST2
• Backbone manufacturer: James Hurley Lab
• Backbone size w/o insert (bp): 4905
• Total vector size (bp): 6694
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OPTN
• Alt name: GLC1E
• Species: H. sapiens (human)
• Mutation: S177D S473D
• GenBank ID: 10133 NM_021980
• Entrez Gene: OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
• Tag / Fusion Protein:
  • GST-TEV (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CATGTCCCCTATACTAGGTTATTGGAAAAT
• 3′ sequencing primer: TTAaatgatgcaatccatcacgtgaatctg

Resource Information

• Supplemental Documents:
  • SMC1588 - pGST2-GST-TEV-hOPTN S177D S473D.gb
• A portion of this plasmid was derived from a plasmid made by: The lab of James Hurley: Plasmid #171425

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry