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p70 delta2-46 delta CT104
(Plasmid #1898)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1898 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 1898-D50 50 μg of DNA in Tris buffer $495
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DNA 1898-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p70 alpha1 S6 kinase
  • Alt name
    p70 S6 kinase
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1300
  • Mutation
    delta2-46, deltaCT104 (deletion of aa 2-46 and amino acids after residue 421)
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p70 delta2-46 delta CT104 was a gift from Joseph Avruch (Addgene plasmid # 1898 ; http://n2t.net/addgene:1898 ; RRID:Addgene_1898)

  • For your References section:

    Multiple independent inputs are required for activation of the p70 S6 kinase. Weng QP, Andrabi K, Kozlowski MT, Grove JR, Avruch J. Mol Cell Biol 1995 May;15(5):2333-40. PubMed 7739516
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