|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8991||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)1500
MutationF5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)
Entrez GeneRps6kb1 (a.k.a. p70 S6K-alpha)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
See Schalm et. al., 2005 JBC Vol. 280(12)11101-11106.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK7-HA-S6K1-F5A-E389-R3A was a gift from John Blenis (Addgene plasmid # 8991 ; http://n2t.net/addgene:8991 ; RRID:Addgene_8991)
For your References section:Identification of a conserved motif required for mTOR signaling. Schalm SS, Blenis J. Curr Biol. 2002 Apr 16. 12(8):632-9. 10.1016/S0960-9822(02)00762-5 PubMed 11967149