pMXs-hSOX2
(Plasmid #193351)

• Purpose: Retroviral vector expressing human SOX2
• Depositing Lab: Hans Schöler
• Publication: MacCarthy et al bioRxiv 2022.09.23.509242

Backbone

• Vector backbone: pMXs
  (Search Vector Database)
• Backbone size w/o insert (bp): 4627
• Total vector size (bp): 5601
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOX2
• Species: H. sapiens (human)
• Insert Size (bp): 974
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)
• Promoter: 5'LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: gacggcatcgcagcttggatacac
• 3′ sequencing primer: CCCTTTTTCTGGAGACTAAATAAAATC

Resource Information

• Supplemental Documents:
  • pMXs-hSOX2.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry