EGFP-HsNRF2 (NFE2L2)
(Plasmid
#194303)
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PurposeMammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH
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Depositing Lab
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Depositing OrganizationUniversity of Queensland, Institute for Molecular Bioscience
Located in Australia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 194303-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 194303-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCSDEST2 (Addgene 22424)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFE2L2
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SpeciesH. sapiens (human)
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Entrez GeneNFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.
DNA (Catalog # 194303-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 194303-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-HsNRF2 (NFE2L2) was a gift from Rob Parton (Addgene plasmid # 194303 ; http://n2t.net/addgene:194303 ; RRID:Addgene_194303) -
For your References section:
Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2. Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG. Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28. 10.1016/j.devcel.2023.02.004 PubMed 36858041
