Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2.
Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG
Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28. PubMed Article
Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG
Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194288 | pME-p3E-GLRX-roGFP2 | Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF |
| 194289 | pME-p3E-HyPer7 | Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV |
| 194290 | p3E-DrCav1 | Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT |
| 194291 | p3E-DrCavin1a | Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM |
| 194292 | p3E-DrCavin1b | Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ |
| 194293 | mKate2-DrCav1 in pT3TS-Dest | In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW |
| 194294 | mKate2-DrCavin1a in pT3TS-Dest | In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI |
| 194295 | mKate2-DrCavin1b in pT3TS-Dest | In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC |
| 194296 | Hyper7 in pT3TS-Dest | In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL |
| 194297 | GLRX-roGFP2 in pT3TS-Dest | In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK |
| 194302 | p3E-HsNRF2 (NFE2L2) | Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS |
| 194303 | EGFP-HsNRF2 (NFE2L2) | Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH |