pGTag NGFR EGFP nLAP -2
(Plasmid
#194507)
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PurposeCRISPR/Cas9-mediated generic protein tagging
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript KS-
- Total vector size (bp) 5229
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Vector typeBacterial Cloning Vector
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Selectable markersNGFR for affinity purification
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNGFR-P2A-nLAP(EGFP)
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Insert Size (bp)1890
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGTag NGFR EGFP nLAP -2 was a gift from Frank Schnütgen (Addgene plasmid # 194507 ; http://n2t.net/addgene:194507 ; RRID:Addgene_194507) -
For your References section:
Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR. Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, Wempe F, Serve H, Schnutgen F. MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. eCollection 2022. 10.1016/j.mex.2022.101929 PubMed 36444289
Map uploaded by the depositor.
