Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR.
Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, Wempe F, Serve H, Schnutgen F
MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. eCollection 2022. PubMed Article
Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, Wempe F, Serve H, Schnutgen F
MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. eCollection 2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194308 | pGTag Hygro EGFP cLAP +0 | CRISPR/Cas9-mediated generic protein tagging |
| 194309 | pGTag Hygro EGFP cLAP +1 | CRISPR/Cas9-mediated generic protein tagging |
| 194310 | pGTag Hygro EGFP cLAP +2 | CRISPR/Cas9-mediated generic protein tagging |
| 194311 | pGTag Hygro EGFP cLAP -0 | CRISPR/Cas9-mediated generic protein tagging |
| 194312 | pGTag Hygro EGFP cLAP -1 | CRISPR/Cas9-mediated generic protein tagging |
| 194313 | pGTag Hygro EGFP cLAP -2 | CRISPR/Cas9-mediated generic protein tagging |
| 194314 | pGTag NGFR EGFP nLAP +0 | CRISPR/Cas9-mediated generic protein tagging |
| 194315 | pGTag NGFR EGFP nLAP +1 | CRISPR/Cas9-mediated generic protein tagging |
| 194316 | pGTag NGFR EGFP nLAP +2 | CRISPR/Cas9-mediated generic protein tagging |
| 194505 | pGTag NGFR EGFP nLAP -0 | CRISPR/Cas9-mediated generic protein tagging |
| 194506 | pGTag NGFR EGFP nLAP -1 | CRISPR/Cas9-mediated generic protein tagging |
| 194507 | pGTag NGFR EGFP nLAP -2 | CRISPR/Cas9-mediated generic protein tagging |
| 194508 | pGTag NGFR EGFP cLAP +0 | CRISPR/Cas9-mediated generic protein tagging |
| 194509 | pGTag NGFR EGFP cLAP +1 | CRISPR/Cas9-mediated generic protein tagging |
| 194510 | pGTag NGFR EGFP cLAP +2 | CRISPR/Cas9-mediated generic protein tagging |
| 194511 | pGTag NGFR EGFP cLAP -0 | CRISPR/Cas9-mediated generic protein tagging |
| 194512 | pGTag NGFR EGFP cLAP -1 | CRISPR/Cas9-mediated generic protein tagging |
| 194513 | pGTag NGFR EGFP cLAP -2 | CRISPR/Cas9-mediated generic protein tagging |