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pGTag Hygro EGFP cLAP +2
(Plasmid #194310)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194310 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 194310-D50 50 μg of DNA in Tris buffer $495
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DNA 194310-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript KS-
  • Total vector size (bp) 5404
  • Vector type
    Bacterial Cloning Vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cLap(EGFP)-P2A-Hygro
  • Insert Size (bp)
    2067

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTag Hygro EGFP cLAP +2 was a gift from Frank Schnütgen (Addgene plasmid # 194310 ; http://n2t.net/addgene:194310 ; RRID:Addgene_194310)

  • For your References section:

    Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR. Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, Wempe F, Serve H, Schnutgen F. MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. eCollection 2022. 10.1016/j.mex.2022.101929 PubMed 36444289
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