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(Plasmid #118055)


Item Catalog # Description Quantity Price (USD)
Plasmid 118055 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested with EcoRI to remove the T2A-Puro cassette, and a P2A-Blasticidin cassette was amplified by PCR, digested with EcoRI, and ligated.

The BbsI site (GAAGAC) in the blasticidin-resistance gene was mutated to GAGGAC with no change in amino acids.

gRNA inserts can be sequenced using pLKO.1 5’ primer, 5’-GACTATCATATGCTTACCGT-3’.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9(BB)-2A-Blast was a gift from Ken-Ichi Takemaru (Addgene plasmid # 118055 ; ; RRID:Addgene_118055)