Purpose(Empty Backbone) Used for expression of sgRNA in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127763||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGCTGGGAGGCGACAAAAGG (Common Sequencing Primers)
Terms and Licenses
pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested with EcoRI to remove the T2A-Puro cassette, and a P2A-Hygromycin cassette was amplified by PCR, digested with MfeI, and ligated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSpCas9(BB)-2A-Hygro was a gift from Ken-Ichi Takemaru (Addgene plasmid # 127763 ; http://n2t.net/addgene:127763 ; RRID:Addgene_127763)