pDest Lenti CA-GFP
(Plasmid #197459)

• Purpose: Bicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.
• Depositing Lab: Omar Khan
• Publication: Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505. doi: 10.3390/cancers15020505.

Backbone

• Vector backbone: pLV[Exp]-Neo-CMV
• Backbone manufacturer: vectorbuilder
• Backbone size w/o insert (bp): 3970
• Total vector size (bp): 4824
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: none.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: or STBL3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: GFP (S65T)
• Species: Synthetic
• Insert Size (bp): 854
• Mutation: S65T
• Promoter: CMV, IRES

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: vectorbuilder.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Designed in Khan lab, manufactured by vectorbuilder.