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Addgene

pYIC
(Plasmid #18673)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18673 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo
  • Species
    H. sapiens (human); Aequorea victoria; synthetic sequence.
  • Insert Size (bp)
    895
  • Tags / Fusion Proteins
    • 3Myc-EYFP-HA-His6 (N terminal on insert)
    • ECFP-HA-His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI and BstXI (not destroyed)
  • 3′ cloning site Eco RI and Not I (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ECFP and EYFP were PCR amplification products from pECFP-Mito and pEYFP-N1 DNAs from Clontech.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EYFP tagged at N-terminus with 3 copies of Myc epitope tags and at C-terminus with a copy of FLAG and polyhistidine (His6) epitope tags. ECFP tagged at the C-terminus with one copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags.

EGFP of pIERS2-EGFP (Clontech) replaced at an 803 bp BstXI-NotI, PCR amplified fragment with ECFP from pECFP-Mito (Clontech) tagged at the C-terminus with a single copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags; IRES-dependent translation yields ECFP-HA-His6 fusion protein. 895 bp Sac I/Eco RI fragment containing amino terminal 3 Myc epitope tags fused to EYFP obtained from PCR amplification of pEYFP-N1 (Clontech), which is also tagged at C-terminus with a single copy of FLAG and polyhistidine (His6) epitope tag; cap-dependent translation yields 3Myc-EYFP-Flag-His6 fusion protein.

See article for detailed information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYIC was a gift from Han Htun (Addgene plasmid # 18673 ; http://n2t.net/addgene:18673 ; RRID:Addgene_18673)
  • For your References section:

    Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR. Nie M, Htun H. Nucleic Acids Res. 2006 . 34(19):5528-40. 10.1093/nar/gkl584 PubMed 17023487