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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 18784-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 18784-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepYIC
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Backbone manufacturerAvailable at Addgene (#18673)
- Backbone size w/o insert (bp) 6270
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-ferritin iron response element
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Alt nameIRE
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Insert Size (bp)35
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An oligonucleotide with one copy of H-ferritin iron response element, IRE, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with IRE: The IRE was inserted at the NheI and SacI sites (pIRE-YIC; 15 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).
DNA (Catalog # 18784-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 18784-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRE YIC was a gift from Han Htun (Addgene plasmid # 18784 ; http://n2t.net/addgene:18784 ; RRID:Addgene_18784) -
For your References section:
Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR. Nie M, Htun H. Nucleic Acids Res. 2006 . 34(19):5528-40. 10.1093/nar/gkl584 PubMed 17023487
Map uploaded by the depositor.
