GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3
(Plasmid #198451)

• Purpose: N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
• Depositing Lab: Annette Dolphin
• Depositing Organization: University College London
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060. doi: 10.1093/function/zqad060. eCollection 2024.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen Life Technologies
• Backbone size w/o insert (bp): 5352
• Total vector size (bp): 5352
• Modifications to backbone: additional restriction sites in MCS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB medium
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cav2.2
• Alt name: calcium channel alpha1B
• Species: H. sapiens (human)
• Insert Size (bp): 7947
• Entrez Gene: CACNA1B (a.k.a. BIII, CACNL1A5, CACNN, Cav2.2, DYT23, NEDNEH)
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal GFP, internal double HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CTGGCTAACTAGAGAACC
• 3′ sequencing primer: ATCAGCGAGCTCAGCAT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene - item ID 62574 - huCaV2.2 (pSAD442-1) ,

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 198451-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 198451-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.