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huCaV2.2 (pSAD442-1)
(Plasmid #62574)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62574 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA6/V5-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 12200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CACNA1B
  • Alt name
    CaV2.2
  • Alt name
    huCaV2.2 (pSAD442-1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7100
  • Entrez Gene
    CACNA1B (a.k.a. BIII, CACNL1A5, CACNN, Cav2.2, DYT23, NEDNEH)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

contains alternate exons (+e10a, +18a, Δ19a, +e31a, +e37b and +e46)

Note that the human CaV2.2 insert contains the native stop codon. The V5 and His tags present in the pcDNA6/V5-HisA backbone are NOT in frame with CaV2.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    huCaV2.2 (pSAD442-1) was a gift from Diane Lipscombe (Addgene plasmid # 62574 ; http://n2t.net/addgene:62574 ; RRID:Addgene_62574)
  • For your References section:

    CACNA1B mutation is linked to unique myoclonus-dystonia syndrome. Groen JL, Andrade A, Ritz K, Jalalzadeh H, Haagmans M, Bradley TE, Jongejan A, Verbeek DS, Nurnberg P, Denome S, Hennekam RC, Lipscombe D, Baas F, Tijssen MA. Hum Mol Genet. 2015 Feb 15;24(4):987-993. Epub 2014 Oct 8. 10.1093/hmg/ddu513 PubMed 25296916