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PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 198735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep601 modified
- Total vector size (bp) 4967
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNpuC-CtermPE2max∆RNaseH
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Insert Size (bp)2850
- Promoter Cbh
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGCGAGTCAGTGAGCGAG
- 3′ sequencing primer GTTTTCCCAGTCACGAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
v3em-Cterm-PE2max-∆RNaseH-dualU6 was a gift from David Liu (Addgene plasmid # 198735 ; http://n2t.net/addgene:198735 ; RRID:Addgene_198735) -
For your References section:
Efficient prime editing in mouse brain, liver and heart with dual AAVs. Davis JR, Banskota S, Levy JM, Newby GA, Wang X, Anzalone AV, Nelson AT, Chen PJ, Hennes AD, An M, Roh H, Randolph PB, Musunuru K, Liu DR. Nat Biotechnol. 2023 May 4. doi: 10.1038/s41587-023-01758-z. 10.1038/s41587-023-01758-z PubMed 37142705
