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Purposelipophagy reporter system based on the Keima-fluorophore
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 199695-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 199695-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 4909
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Modifications to backbone+Keima-LiveDrop
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGPAT4
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Alt nameAGPAT6; LPAATZ; TSARG7; 1-AGPAT 6; LPAAT-zeta
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SpeciesD. melanogaster (fly)
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Insert Size (bp)171
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Entrez GeneGpat4 (a.k.a. Dmel_CG3209, CG3209, DmGPAT3/4, Dmel\CG3209, GPAT, GPAT4, dGPAT4)
- Promoter CMV
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Tag
/ Fusion Protein
- Keima (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI, BmtI (unknown if destroyed)
- 3′ cloning site PspXI, PaeR7I, XhoI (unknown if destroyed)
- 5′ sequencing primer GCACAGCTAGCGCCACCATGGTGAGCGTGATCGCC
- 3′ sequencing primer GCACACTCGAGCGCCCAGCAGGGAGTGGCGGGCGATG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 199695-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 199695-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKeima-LiveDrop was a gift from Tobias Walther (Addgene plasmid # 199695 ; http://n2t.net/addgene:199695 ; RRID:Addgene_199695) -
For your References section:
The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets. Chung J, Park J, Lai ZW, Lambert TJ, Richards RC, Zhang J, Walther TC, Farese RV Jr. Nat Cell Biol. 2023 Aug;25(8):1101-1110. doi: 10.1038/s41556-023-01178-w. Epub 2023 Jul 13. 10.1038/s41556-023-01178-w PubMed 37443287
Map uploaded by the depositor.
