pmKO-imp α
(Plasmid
#200082)
-
PurposeExpresses mKO-tagged human importinα in mammalian cells
-
Depositing Lab
-
Depositing OrganizationOsaka Metropolitan University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 200082-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 200082-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepmKO1-MC1
-
Backbone manufacturerMBL
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 5700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameimportin α
-
Alt nameKPNA2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1000
-
Entrez GeneKPNA2 (a.k.a. IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha)
- Promoter CMV
-
Tag
/ Fusion Protein
- mKO (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer mKO C-terminal side
- 3′ sequencing primer SV40 poly A side
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The DNA fragment of imp α is the same as the one cloned in pEGFP-imp α
Plasmid vector pmKO1-MC1 is the same as the pmKO-AF8 except for the cloning sites.
DNA (Catalog # 200082-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 200082-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmKO-imp α was a gift from Kenji Sugimoto (Addgene plasmid # 200082 ; http://n2t.net/addgene:200082 ; RRID:Addgene_200082) -
For your References section:
A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin alpha as fusion tag. Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7. 10.1271/bbb.110677 PubMed 22313767
