pME map2k2a_s219D
(Plasmid
#201058)
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PurposeGateway middle entry vector expressing constitutively active zebrafish map2k2a
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerThermoFisher
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 5956
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAP2k2a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1194
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MutationMutation S219D, constitutively active
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GenBank IDNM_001037391
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Entrez Genemap2k2a (a.k.a. fa56c06, map2k2, mek2, wu:fa56c06, zgc:123171)
- Promoter none
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13_forward
- 3′ sequencing primer m13_reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME map2k2a_s219D was a gift from Sarah Childs (Addgene plasmid # 201058 ; http://n2t.net/addgene:201058 ; RRID:Addgene_201058) -
For your References section:
rasa1-related arteriovenous malformation is driven by aberrant venous signalling. Greysson-Wong J, Rode R, Ryu JR, Chan JL, Davari P, Rinker KD, Childs SJ. Development. 2023 Sep 15;150(18):dev201820. doi: 10.1242/dev.201820. Epub 2023 Sep 14. 10.1242/dev.201820 PubMed 37708300
