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(Plasmid #86119)


Item Catalog # Description Quantity Price (USD)
Plasmid 86119 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Modifications to backbone
    Replaced pETDuet-1 T7 promoters and ORFs with: pTlpA and pLacI pR/pL and pLacI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    S. Typhimurium
  • Insert Size (bp)
  • Mutation
    D341A, P60L, D135V, K187R, K202I, L208Q
  • GenBank ID
  • Promoter pTlpA, pLacI

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCGTTTACGAGATC
  • 3′ sequencing primer gcctcactgattaagcattgg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Promoter pTlpA
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGCGTCCGGCGTAGA
  • 3′ sequencing primer gccagccctgcttcaata
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
  • Alt name
  • Alt name
    Lambda Repressor
  • Insert Size (bp)
  • Mutation
  • Promoter pR/pL, pLacI

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aagttggacatcacctcc
  • 3′ sequencing primer ACTACTGGGCTGCTTCC
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
  • Promoter pR/pL

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTGAGCACATCAGCAGG
  • 3′ sequencing primer gtgttaatGGTTTCTTTTTTGTGCTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCali2 was a gift from Mikhail Shapiro (Addgene plasmid # 86119 ; ; RRID:Addgene_86119)
  • For your References section:

    Tunable thermal bioswitches for in vivo control of microbial therapeutics. Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG. Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14. 10.1038/nchembio.2233 PubMed 27842069