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Tunable thermal bioswitches for in vivo control of microbial therapeutics.
Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG
Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85455pTlpA-WasabimWasabi reporter gene gated by wild type TlpA
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86100pTlpA-Wasabi-NFNonfluorescent variant of mWasabi reporter gene gated by wild type TlpA
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86101pTcI-WasabimWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI
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86102pLacI241-WasabimWasabi reporter gene gated by temperature-sensitive LacI A241T
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86103pLacI265-WasabimWasabi reporter gene gated by temperature-sensitive LacI G265D
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86104pTetR89-WasabimWasabi reporter gene gated by temperature-sensitive TetR A89D
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86105pTetR193-WasabimWasabi reporter gene gated by temperature-sensitive TetR I193N
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86107pRpoH-WasabimWasabi reporter gene driven by RpoH promoter
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86108pClp-WasabimWasabi reporter gene driven by ClpP/ClpX promoter
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86109pHtpG-WasabimWasabi reporter gene driven by HtpG promoter
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86110pDnaK-WasabimWasabi reporter gene driven by DnaK promoter
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86111pGrpE-WasabimWasabi reporter gene driven by GrpE promoter
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86112pLacIq-WasabimWasabi reporter gene driven by LacIq promoter
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86113pTlpAsp-WasabimWasabi reporter gene driven by TlpA promoter with scrambled Pribnow box
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86114pTlpAreverse-WasabimWasabi reporter gene driven by TlpA promoter in reverse orientation
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86115pTlpA36-WasabimWasabi reporter gene gated by TlpA36
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86116pTlpA39-WasabimWasabi reporter gene gated by TlpA39
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86117pTcI38-WasabimWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38
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86118pTcI42-WasabimWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42
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86119pCali2Multiplex temperature-gated expression of mWasabi and mCherry reporters
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86120pThermeleonBandpass temperature-gated expression of mWasabi and mCherry reporters
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