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pLacI265-Wasabi
(Plasmid #86103)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86103 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    T7 Promoter #1 replaced with T7A1O4/O3 T7 Promoter #2 deleted
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LacIts
  • Alt name
    Lac Repressor (Temperature Sensitive)
  • Insert Size (bp)
    1083
  • Mutation
    G265D
  • Promoter pLacI

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer ACTACTGGGCTGCTTCC
  • 3′ sequencing primer gttaagggcggttttttcc
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mWasabi
  • Insert Size (bp)
    756
  • GenBank ID
    EU024648.1
  • Promoter T7A1O4/O3
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer ATGCGTCCGGCGTAGA
  • 3′ sequencing primer CTAGTTATTGCTCAGCGGTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacI265-Wasabi was a gift from Mikhail Shapiro (Addgene plasmid # 86103 ; http://n2t.net/addgene:86103 ; RRID:Addgene_86103)
  • For your References section:

    Tunable thermal bioswitches for in vivo control of microbial therapeutics. Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG. Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14. 10.1038/nchembio.2233 PubMed 27842069