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pTlpAreverse-Wasabi
(Plasmid #86114)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86114 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    T7 Promoter #1 replaced with TlpA promoter (with scrambled Pribnow box) T7 Promoter #2 deleted
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TlpA
  • Species
    S. Typhimurium
  • Insert Size (bp)
    1116
  • Mutation
    D341A
  • GenBank ID
    M88208.1
  • Promoter TlpA (in reverse orientation)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mWasabi
  • Species
    Synthetic
  • Insert Size (bp)
    756
  • GenBank ID
    EU024648.1
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTlpAreverse-Wasabi was a gift from Mikhail Shapiro (Addgene plasmid # 86114 ; http://n2t.net/addgene:86114 ; RRID:Addgene_86114)
  • For your References section:

    Tunable thermal bioswitches for in vivo control of microbial therapeutics. Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG. Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14. 10.1038/nchembio.2233 PubMed 27842069