pMAH-Golgi-bapaUGAc
(Plasmid
#201675)
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Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi
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Depositing Lab
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Depositing OrganizationUniversity of Virginia
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 201675-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 201675-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.0
- Backbone size w/o insert (bp) 6981
- Total vector size (bp) 8105
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGolgi-bapaUGAc
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SpeciesSynthetic
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Insert Size (bp)944
- Promoter CMV SP6
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Tag
/ Fusion Protein
- Golgi leading sequence (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6: CAT TTA GGT GAC ACT ATA G
- 3′ sequencing primer BGHr: TAG AAG GCA CAG TCG AGG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.03.01.530644v1 for bioRxiv preprint.
DNA (Catalog # 201675-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 201675-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAH-Golgi-bapaUGAc was a gift from Huiwang Ai (Addgene plasmid # 201675 ; http://n2t.net/addgene:201675 ; RRID:Addgene_201675) -
For your References section:
Genetically Encoded Boronolectin as a Specific Red Fluorescent UDP-GlcNAc Biosensor. Zhang J, Li Z, Pang Y, Fan Y, Ai HW. ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409. 10.1021/acssensors.3c00409 PubMed 37480329
