Skip to main content
Addgene
Open Menu

pUC19/PLIN2-GFP
(Plasmid #202194)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202194 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 202194-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 202194-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4600
  • Modifications to backbone
    added C-terminal PLIN2 homology arms
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLIN2
  • Alt name
    GFP knock-in to C-terminus of PLIN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1897
  • GenBank ID
    NM_001122.4
  • Entrez Gene
    PLIN2 (a.k.a. ADFP, ADRP)
  • Promoter lac
  • Tag / Fusion Protein
    • GFP knock-in

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13/pUC Reverse
  • 3′ sequencing primer M13 (-21) Forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19/PLIN2-GFP was a gift from James Olzmann (Addgene plasmid # 202194 ; http://n2t.net/addgene:202194 ; RRID:Addgene_202194)

  • For your References section:

    Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation. Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA. Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001. 10.1016/j.devcel.2023.07.001 PubMed 37494933
Related items:
Commonly requested with: