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PEmax-mRNA
(Plasmid #204472)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204472 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 204472-D50 50 μg of DNA in Tris buffer $495
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DNA 204472-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pbluescript
  • Backbone manufacturer
    NA
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 9167
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prime editor
  • Alt name
    PEmax mRNA
  • Species
    human
  • Promoter T7
  • Tag / Fusion Protein
    • no

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PEmax-mRNA was a gift from Scot Wolfe (Addgene plasmid # 204472 ; http://n2t.net/addgene:204472 ; RRID:Addgene_204472)

  • For your References section:

    Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency. Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Maitland SA, Lawson ND, Luban J, Wolfe SA. Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456. 10.1093/nar/gkad456 PubMed 37246708
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