MPRA_minCMV+GFP
(Plasmid
#204866)
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PurposeVector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.
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Depositing Lab
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Depositing OrganizationHospital for Sick Children
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 204866-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 204866-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL4.23
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2642
- Total vector size (bp) 3481
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameminCMV:GFP
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Alt namepeTurboGFP-dest1
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SpeciesPontellina plumata
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Insert Size (bp)839
- Promoter minimal CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CGCCGCGTGGAGGAGGA
- 3′ sequencing primer CGACGCTCTTCCGATCT
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRinn Lab, CU Boulder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 204866-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 204866-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MPRA_minCMV+GFP was a gift from Philipp Maass (Addgene plasmid # 204866 ; http://n2t.net/addgene:204866 ; RRID:Addgene_204866) -
For your References section:
Systematic characterization of regulatory variants of blood pressure genes. Oliveros W, Delfosse K, Lato DF, Kiriakopulos K, Mokhtaridoost M, Said A, McMurray BJ, Browning JWL, Mattioli K, Meng G, Ellis J, Mital S, Mele M, Maass PG. Cell Genom. 2023 May 24;3(7):100330. doi: 10.1016/j.xgen.2023.100330. eCollection 2023 Jul 12. 10.1016/j.xgen.2023.100330 PubMed 37492106
Map uploaded by the depositor.
