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pCAIP-hXRCC1wt-eGFP
(Plasmid #206032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206032 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 206032-D50 50 μg of DNA in Tris buffer $495
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DNA 206032-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRES-puro
  • Backbone manufacturer
    Clonetec
  • Backbone size w/o insert (bp) 4476
  • Total vector size (bp) 8880
  • Modifications to backbone
    original CMV promoter was exchanged with a CAG promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hXRCC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4041
  • Promoter CAG
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCAGGS-5
  • 3′ sequencing primer EGFP_N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Angelika Jacobs and David Schürmann from the Schär lab created this plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAIP-hXRCC1wt-eGFP was a gift from Primo Schaer (Addgene plasmid # 206032 ; http://n2t.net/addgene:206032 ; RRID:Addgene_206032)

  • For your References section:

    Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, Slupphaug G, Hottiger MO, Schar P, Steinacher R. Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8. 10.1038/s41467-023-44209-8 PubMed 38167803
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