pCAIP-hXRCC1pd-eGFP
(Plasmid
#206033)
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PurposeMammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter
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Depositing Lab
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Depositing OrganizationUniversity of Basel
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 206033-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 206033-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRES-puro
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Backbone manufacturerClonetec
- Backbone size w/o insert (bp) 4476
- Total vector size (bp) 8880
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Modifications to backboneoriginal CMV promoter was exchanged with a CAG promoter
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehXRCC1
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SpeciesH. sapiens (human)
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Insert Size (bp)4041
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MutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A
- Promoter CAG
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Tag
/ Fusion Protein
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoNI (not destroyed)
- 5′ sequencing primer pCAGGS-5
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bythe sequence containing the mutated PAR-acceptor sites and mentioned restriction sites was ordered with Twist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 206033-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 206033-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAIP-hXRCC1pd-eGFP was a gift from Primo Schaer (Addgene plasmid # 206033 ; http://n2t.net/addgene:206033 ; RRID:Addgene_206033) -
For your References section:
Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, Slupphaug G, Hottiger MO, Schar P, Steinacher R. Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8. 10.1038/s41467-023-44209-8 PubMed 38167803
