pJS61
(Plasmid
#207585)
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PurposeGFP-TolA expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP-TolA
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SpeciesE. coli
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Insert Size (bp)2019
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGerding et al., 2007
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJS61 was a gift from Colin Kleanthous (Addgene plasmid # 207585 ; http://n2t.net/addgene:207585 ; RRID:Addgene_207585) -
For your References section:
Tunable force transduction through the Escherichia coli cell envelope. Williams-Jones DP, Webby MN, Press CE, Gradon JM, Armstrong SR, Szczepaniak J, Kleanthous C. Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2306707120. doi: 10.1073/pnas.2306707120. Epub 2023 Nov 16. 10.1073/pnas.2306707120 PubMed 37972066
